Immunohistological Analysis of Tissue Specificity of the Fifty-two Workshop Anti-B Lymphocyte Monoclonal Antibodies

Abstract

For the Second International Workshop on Human Leukocyte Differentiation Antigens the 52 monoclonal antibodies in the anti-B cell panel were investigated, attaching particular importance to the demonstration of the tissue localization of antigens recognized by the antibodies, and to the selection of antibodies that might be valuable in immunohistological techniques. Because of limitations in our laboratory resources and in time, only such Workshop antibodies that were selected by a screening procedure were selected for more extensive study in the search for antibodies recognizing B cell differentiation stages, and in the search for clustering of antibody reactivity patterns. For this purpose, frozen biopsies of B cell lymphomas of well-established histological subcategories which had already been extensively phenotyped with other monoclonal antibodies were used. In addition, a few cases of T cell lymphomas, Hodgkin’s disease, and polymorphic large-cell Ki-1-positive lymphoma (“malignant histiocytosis”) were also included in the extended study. Finally, all the selected anti-B cell antibodies were tested for reactivity in sections of formalin-fixed and paraffin-embedded tissue. The results are presented in the following.