Abstract
Publisher Summary This chapter explores the immunohistochemical localization of NADPH-dependent cytochrome C reductase in liver, lung, and kidney of phenobarbital versus saline-pretreated rats. In an experiment described in the chapter, in the liver, specific immunofluorescence was seen in hepatocyte cytoplasm but not in the nuclei and staining was stronger in centrilobular than in periportal zones. Livers from phenobarbital rats showed a more intense and consolidated pattern of staining in the hepatocyte cytoplasm than livers from saline-rats. This correlates well with the two- to threefold increase in NADPH-cytochrome c reductase activity seen biochemically and with the proliferation of smooth-surfaced endoplasmic reticulum seen by electron microscopy. Preliminary immunoelectron microscopy suggests that ferritin-labeled antibody is located around areas of smooth-surfaced endoplasmic reticulum in these animals. Immunofluorescence was weak to negative in endothelial and connective tissues in both pretreatments. Cytochrome P-450 content increased 4 to 5 times while cytochrome b5 content showed minimal change. Aminopyrine, benzphetamine, benzo(a)pyrene, and laurate metabolism increased in liver microsomes from the phenobarbital-pretreated animals. These results agree with the immunohistochemical studies and with established biochemical and ultrastructural observations on the effects of phenobarbital.
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