Immunoglobulin class of antibodies in monkeys infected with Toxocara canis

Abstract

Sera of monkeys ( Macaca sinica), experimentally infected with multiple doses of eggs of Toxocara canis, were subjected to Sephadex G-200 gel filtration and DEAE cellulose ion exchange chromatography. The distribution of antibodies in the various serum fractions was determined by the conglutinating complement absorption test (CCAT) and the agar-gel diffusion precipitin test using antigens prepared from the adult and the embryonated eggs of T. canis and by the formation of precipitates at the oral orifice of artificially hatched living 2nd stage larvae of T. canis. In serum samples taken 1 and 2 weeks after initial infection, CCAT antibodies were located mainly in the macroglobulin fractions of serum. By 1 to 4 weeks after a third dose of eggs such antibodies were located mainly in the 7S fraction of immunoglobulins. Antibodies detected by gel precipitation were located in the macroglobulin fraction of serum, while those that produced oral precipitates on 2nd stage larvae occurred in the 7S fractions.