Abstract
Three water-soluble polysaccharides (DDP1-1, DDP2-1 and DDP3-1) were obtained from the aqueous extracts of the stems of Dendrobium denneanum by hot water extraction, ethanol precipitation, and fractionated by DEAE-cellulose ion exchange chromatography and Sephadex G-200 gel filtration chromatography. The main structure characterizations and anti-tumor activities of three fractions were evaluated in this paper. The results showed DDP1-1 with 12.50 mg/kg could significant inhibit the increment of the tumor, increase immune index of S180 mice, and also strongly promote the secretion of IL-2, TNF-α and IFN-γ. Therefore, results of these studies demonstrated the polysaccharide DDP1-1 has strong anti-tumor and immunomodulation abilities. Key words: Immunomodulating, Dendrobium denneanum, polysaccharide, anti-tumor.
Highlights
Dendrobium denneanum, belongs to the Orchidaceae family, a traditional Chinese herb, has been used in the preparation of herbal medicines in many oriental countries for a long time
Because the dried stems could be used widely for the treatment of salivary, ophthalmic disorders, fever and chronic superficial gastritis, D. denneanum has been archived in the Pharmacopoeia of the People's Republic of China
The tumor necrosis factoralpha (TNF- ), interferon-gamma (IFN- ) and interleukin-2 (IL-2) enzyme-linked immunosorbent assay (ELISA) kits were purchased from Shanghai Senxiong Biotech Co. (Shanghai, China)
Summary
Dendrobium denneanum, belongs to the Orchidaceae family, a traditional Chinese herb, has been used in the preparation of herbal medicines in many oriental countries for a long time. Polysaccharides is some of its main bioactive compounds that possesses immunoregulatory (Shao et al, 2004; Chen et al, 2007; Feng et al, 2010), anti-inflammatory (Hitoshi et al, 1974; Popov et al, 2005), anti-virus (Wang et al, 2009) and antioxidant (Wu et al, 2007; Luo et al, 2009, 2010) activities. Plant polysaccharides are ideal candidates for therapeutics with immunomodulatory and antitumor effects and low toxicity (Schepetkin et al, 2006). In order to investigate the bioactive of the polysaccharides from D. denneanum, the isolated and purified procedure and antitumor and immunopotentiating activities using a tumor inhibitory assay system and immunepotentiation assay system were carried out in the present study
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