Abstract
Specific antibodies against glucosylceramide were elicited in rabbits by repeated intravenous injection of a mixture of hapten-cholesterol-human albumin (0.2:1:4) (mg/ml). The antisera obtained were titrated both by complement fixation and by passive agglutination. They exhibited little cross-reactivity with galactosylceramide and non with sulfatide. Among various sugars tested, methyl glucoside was found the most efficient to inhibit the antibody's reactivity. The antiglucosylceramide antibodies reacted with the natural hapten present in sheep red blood cell membranes as shown by indirect immunofluorescence.
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