Immunogenic antigens from Streptococcus mutans which stimulate secretory IgA response from parotid saliva in children with caries

Abstract

The aim of this study was identify immunogenic antigens from S. mutans which stimulate secretory IgA response from parotid saliva with caries in children.The sample consisted of 55 children aged 6-10 years old with caries, they took a sample of unstimulated parotid saliva with Schaefer capsules. Cultured S. mutans ATCC 35668, the bacteria were disintegrated by sonication, electrophoresis was performed proteins obtained from the bacterium and transferred to PVDF membrane and conducted western blot analysis the collected saliva. Unstimulated parotid saliva of children with caries containing IgA antibodies reactive to antigens of S. mutans of 26 and 19 kDa and there IgA reactivity against 190 kDa antigen (Ag I/II) in about half of the collected sample 26/55 (47%). Unstimulated parotid saliva of children with caries containing IgA antibodies reactive to antigens of S. mutans of 26 and 19 kDa. IgAs reactivity against 190 kDa antigen (Ag I / II) in about half of the collected sample (26 children, n = 55). IgAs reactivity against 250 kDa antigen in the middle of the collected sample (28/55). Which may be the antigen SpA of S. sobrinus (210 kDa) and cross-reactive to the antigen of S. mutans.