Abstract
SUMMARYA monoclonal antibody (Mab 2a8) has been generated against a DNA tight binding protein of eukaryotic cells. Its distribution was investigated in salivary gland polytene chromosomes of two Chironomid species, Endochironomus tendens and Glyptotendipes gripekoveni, by immunofluorescence. The results indicate: 1. Mab 2a8 is distributed in some distinct condensed bands along the entire lenght of chromosomes; 2. The banding pattern obtained with fluorescent antibody does almost strictly correspond to some Hoechst positive bands and to constitutive heterochromatin sites established by «C» banding method. Other condensed (Hoechst positive) bands on chromosomes were Mab 2a8 immunonegative. 3. No immunofluorescent was observed in genetically active regions of polytene chromosomes (puffs, Balbiani rings and nuclear organizer). The mode of distribution in defined bands of polytene chromosomes suggests the residence of this protein in polytene chromosomes, whose presence appears to be well in correlation with t...
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