Immunofluorescence Identification of the Endocrine Cells in the Pancreatic Islets of the Camel (Camelus dromedarius)

Abstract

The aim of the study and characterize the spread of two types of endocrine cells of the pancreatic alpha and beta with hormones in one hump camel (Camelus dromedaries ) by using immunofluorescence technique and compositions histological study of the pancreas. Conducted the study on the pancreas to twelve of the camels from AL-Dwaynia province slaughter house , six animal for histological study and six other for immunofluercent study . To identify two types of endocrine cells in the pancreas with endocrine hormones. By use specific antisera multiple glucagon peptide-1 and Glucose-dependent insulinotropic peptide. The results showed that the distribution of these types of immune cells in the pancreas difference. Immunoreactive cells (beta) cells, the most numerous and take a central position and surround the pancreatic islets . The most distinct delineation of the islets from the surrounding acini. Glucose-dependent insulinotropic peptide -immunoreactive cells (β-cells) were most numerous followed by Glucagon like peptide-1 (α-) -immunoreactive cells in decreasing order . Alpha cells were mostly observed as clumps in the periphery area. The result concluded from the current study, the difference in the distribution of these cells depends on the type of species when compared to the results of this study with other results.