Abstract
Specific antibodies were prepared against rat-brain UDP-galactose:ceramide galactosyltransferase (CGalT) and used to study the localization of this enzyme at light and electron microscopic levels. Using an immunocytochemical technique the presence of CGalT was revealed in the cytoplasm and processes of oligodendrocytes and in myelin sheaths of developing and adult rat brain. No immunostaining was detected in neurons or astrocytes. At the ultrastructural level the immunostaining of oligodendrocytes was most intense at the periphery of cytoplasm and probably included plasma membrane. Among the intracellular organelles of oligodendrocytes, specific labelling was occasionally seen in the stacks of Golgi apparatus membranes. In myelin sheaths anti-CGalT staining seems to be restricted to the outermost and innermost lamellae. The finding of CGalT in distant portions of oligodendrocyte processes and in loosely wrapped myelin membranes might indicate that myelin galactocerebrosides are synthesized in the proximity of the site of their incorporation into the newly formed myelin.
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