Abstract
The reaction of anti poly rI·poly rC sera with polynucleotides, polynucleotide complexes and RNA was studied by quantitative precipitin analysis. Differences in the reactivity of two polyribonucleotide complexes poly rA·poly rU and poly rG·poly rC on the one hand, and on the other hand between two complexes containing the same bases, poly rG·poly rC and poly dG·poly dC, were observed. The fact that poly dG·poly dC reacts better with anti poly rI·poly rC antibodies than does poly rG·poly rC is evidence in favor of the hypothesis that the specificity of the antibodies depends especially on the conformation of the double helix, rather that on the presence of a given base or of the nature of the sugar. The specificity of the antibodies for double stranded structures is further emphasized by the low reactivity of polynucleotides not involved in complementary hydrogen bonds. However, preliminary inhibition studies with oligoinosinicacids have given results in accord with the hypothesis of a minor population of antibodies with a different specificity. Anti poly I·poly C antibodies cross-react with RNA and particularly with reovirus RNA. They also react, although to a different extent, with ribosomal and transfer RNA. from mammalian cells. Absorption studies with polynucleotides, polynucleotide complexes and reovirus RNA have given results allowing the conclusion that it is above all the regions of associated base pairs which are recognized by the anti poly I·poly C antibodies in the RNA.
Published Version
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