Abstract
Abstract A synthesized metal-complex compound with catalytic activity for a chemiluminescence reaction has been used for the first time as a labeling reagent for immunoassay. Several complex compounds of iron(III) and cobalt(II) were synthesized, and their catalytic activities for the chemiluminescence reaction between 5-amino-2,3-dihydro-1,4-phthalazinedione (luminol) and hydrogen peroxide (H2O2) were examined. Iron(III) 4,11,18,25-tetracarboxyphthalocyanine (TCP-Fe(III)) showed the highest catalytic activity. The immunoassay of free albumin was carried out by a competitive method using albumin labeled with TCP–Fe(III) by means of the carbodiimide method; free albumin in the range of 2 to 1000 μg/tube could be determined with a coefficient of variation of from 6 to 16% in 5 analyses.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
