Abstract
Objective To perform traditional blood and immunologic examinations for the pathogenesis of immune-induced aplastic anemia(AA) IRM-2 mice model. Methods IRM-2 mice were divided into three groups, namely, the control, radiation, and AA model groups. IRM-2 inbred mice in the AA model group were used to set up the AA model by whole body 137Cs γ irradiation and eye venous plexus injection with DBA/2 mice thymus cells. After 15 days of radiation, blood and bone marrow cells were counted, and immunologic detection was performed. Results The peripheral WBC, RBC, bone marrow cells, and reticulocyte[(2.38±0.53)×109/L,(8.22±0.21)×1012/L,(7.14±1.19)×106/femur, and(50.2±8.9)‰] in the AA model group were lower than those of the control group [(8.23±0.41)×109/L,(10.24±0.91)×1012/L,(16.5±1.61)×106/femur, and (76.2±9.7)‰]. CD4+ cells [(8.91±2.55)%] of the AA model group were lower than those of the control group[(16.14±3.09)%], whereas CD8+ cells [(13.55±2.12)%] were higher than those of the control group [(6.83± 1.14)%]. Peripheral blood and immunological indexes of the AA model group were statistically different from those of the control group. Conclusion IRM-2 mice AA model was successfully established as a good model for the study of AA. Key words: Anemia, aplastic; Models, animal; Immune induction; Evaluation studies
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