Immobilized Enzyme Reactor in On-line LC and Its Application in Drug Screening

Abstract

This study is to give a brief introduction of immobilized enzyme reactor (IMER) in on-line LC and its application in drug screening. The literature of immobilization techniques, immobilization supports and determination of immobilized enzyme activity were reviewed; the application in the drug screening is briefly introduced. It was found that IMER increased the enzymatic stabilization, strikingly shortens reaction time and can be used to perform fast screening of enzyme inhibitor. IMER has wide fields in drug screening application.