Abstract
Immobilization of amyloglucosidase ( AMG) and Zymomonas mobilis cells was studied in order to produce ethanol from sago starch economically. Among various immobilization methods tested, a coimmobilized system using chitin and sodium alginate appeared most promising with respect to ethanol productivity and operational stability. When the system was run in the continuous simultaneous saccharification and fermentation ( SSF) mode, the maximum ethanol productivity was found to be 72.2 g l −1 h −1 at a dilution rate of 3.28 h −1. This system could be run in the stable manner over 40 days with a steady-state ethanol concentration of 44 g l −1 and an ethanol conversion yield of 93%.
Published Version
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