Abstract
Urease was immobilized in conducting polypyrrole and block copolymers of thiophene-capped poly(methyl methacrylate) matrices by electropolymerization. Immobilization of the enzyme was achieved by application of 1.0 V constant potential in a solution of 0.02 M pyrrole, 2 mg/ml urease, 0.5 mg/ml supporting electrolyte. The optimum immobilization conditions, electrolysis time, electrolysis medium, enzyme concentration during electrolysis, type of supporting electrolyte were determined. The kinetic behaviors of free and immobilized urease were determined.
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