Immobilization of GOX on PEG/fluoresence functionalized nanographene oxide to describe fluctuation of glucose level

Abstract

Enzyme, produced and worked in all living things, could work as macromolecular biological catalysts in diverse biochemical processes with particular specificity, like glucose oxidase (GOX). The efficient use of enzyme properties has great importance in pharmaceutics and therapeutics. In this work, we could fabricate naive and effective electrochemical biosensors in the determination of glucose levels via utilizing GOX. Graphene oxide, as a water-soluble derivative of graphene, has shown great promise in a variety of biomedical applications including biosensors. Thus, we established a new-type special platform for GOX immobilization to perform its prosperities, in which nanographene oxide (nGO) was employed as an ideal base and poly(ethylene glycol) (PEG) was conjugated on the edge of nGO sheets to enhance its biocompatibility. Additionally, preferable functional dyes (Rhodamine B/fluorescein isothiocyanate) were also introduced to the platform. Enzyme-nanocomposites were then provided by locating GOX on the platform, i.e., GOX@nGO-PEG-RhB and GOX@nGO-PEG-FITC. The microstructure and composite of platforms and enzyme-nanocomposites were confirmed by diverse characterizations. Finally, on account of corresponding cyclic voltammetric and typical ready-state amperometric curves, it was informed that GOX@nGO-PEG-RhB and GOX@nGO-PEG-FITC could effectively respond to the fluctuation of glucose level as electrochemical biosensor. The present work presents special platforms for the immobilization of enzymes like GOX and provides new-type biosensors in the detection of glucose levels.