Scanning electrochemical microscopy and electrochemical impedance spectroscopy-based characterization of perforated polycarbonate membrane modified by carbon-nanomaterials and glucose oxidase

Abstract

In this research, scanning electrochemical microscopy (SECM) and electrochemical impedance spectroscopy (EIS) were applied for the evaluation of surface characteristics of electrochemical (amperometric) biosensor based on two composite structures consisting of perforated polycarbonate membrane modified by carbon-nanomaterials and glucose oxidase. The first structure consisted of the polycarbonate filter membrane, punctured by 400 nm holes (PCM) consequently modified with single walled carbon nanotubes (SWCNT) and graphene oxide (GO) layer (PCM/SWCNT/GO); and the other structure consisted of the same PCM, consequently modified with SWCNT and reduced graphene oxide (rGO) layer (PCM/SWCNT/rGO). These two composite structures were developed and applied in biosensor design. In order to form a biosensing surfaces the PCM/SWCNT/GO and PCM/SWCNT/rGO composite structures were modified by immobilized glucose oxidase (GOx). The surface of GOx modified PCM/SWCNT/GO composite structure (PCM/SWCNT/GO/GOx) was evaluated by SECM redox-competition mode at different glucose concentrations. It was determined that PCM/SWCNT/GO/GOx composite structure is suitable for the design of amperometric glucose biosensors, because SWCNT/GO layer facilitates electron transfer from GOx via the oxidation of formed catalyzed reaction product H 2 O 2 . Therefore, the PCM/SWCNT/GO/GOx structure is a promising material for the design of amperometric glucose biosensors and biofuel cells, which are useful for a wide variety of environmental purposes and green energy production using food-industry waste. • Punctured polycarbonate membrane (PCM) was modified by single walled carbon nanotubes (SWCNT). • PCM/SWCNT-based structure was modified by graphene oxide (GO) or reduced graphene oxide (rGO). • On the surface of PCM/SWCNT/GO and PCM/SWCNT/rGO structures glucose oxidase (GOx) was immobilized. • Scanning electrochemical microscopy (SECM) and impedance spectroscopy (EIS) were applied. • It was determined that PCM/SWCNT/GO/GOx structure is suitable for the design of glucose biosensor.