Abstract
Enzyme immobilization on a suitable support is one of the strategies used to improve enzyme stability and recovery. In present communication, PVA-CoF2O4 nanocomposite film has been used as a support to develop highly active, stable and magnetically separable immobilized diastase. For preparing magnetically separable support, first Cobalt ferrite nanoparticles have been prepared by using co-precipitation method. Prepared nanoparticles have been dispersed in polyvinyl alcohol (PVA) solution to get a PVA-CoFe2O4 film. The detail characterization of cobalt ferrite nanoparticles and PVA-CoFe2O4 film has been carried out. Enzyme diastase has been immobilized on the PVA-CoFe2O4 film and the catalyst activity of immobilized enzyme has been tested in hydrolysis of starch to maltose. The stability of immobilized diastase has found to be higher than free enzyme. Reusability study of the immobilized diastase has been carried out. Improved stability and reusability of immobilised diastase offer the promising applicability as magnetically separable biocatalyst for industry.
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