Abstract
Cellulase is an enzyme commonly used to break down cellulose to beta-glucose. The demand of cellulase has been arisen due to its extensive range of applications in the industries such as detergents, foods and biofuel production. In present study, cellulase from Trichoderma reesei was immobilized on a functionalized multiwall carbon nanotube (MWCNTs) by physical adsorption method. Fourier Transform Infrared (FTIR) was used to confirm the successful functionalization of MWCNTs and immobilization of cellulase. In this approach, effects of pH and temperature for both free and immobilized cellulase were evaluated. Practically, the immobilized cellulase showed an improvement in pH and thermal stability compared to free cellulase at optimum condition of 50°C and pH 5 with 12% and 8% of increments respectively. For reusability study on CMC analysis, the bionanoconjugate retained 60% of its initial activity even after 3rd cycle of analysis. This feature is beneficial to the industrial applications because of its potential to be easily separated from the end product at the end of the reaction, reuse for several times and helps in development of multiple enzyme reaction systems.
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