Abstract
Cholesterol-dependent cytolysins such as Perfringolysin O (PFO) lyse cells by forming large pores in the target cell membrane; they contribute to infections ranging from food poisoning to pneumonia and listeriosis. PFO monomers bind the target membrane and then oligomerize via a pre-pore intermediate to form pores of 20-50 subunits. Here we study the assembly of these pores using single-molecule fluorescence imaging in Droplet Interface Bilayers. We track the increase in brightness as monomers assemble to form individual pores. We observe significant fluctuations in the number of subunits that occur during the assembly of an individual pore.
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