Abstract
Microscopic chemical mapping of living plant tissues without the use of extrinsic labels would represent a major advance in analytical capability for many areas of biological research; Coherent Raman Scattering (CRS) microscopy offers label-free chemical imaging based on vibrational spectroscopy and is an obvious solution. However, due to the high levels of optical absorption and fluorescent emission in plant tissues the technique is severely limited for in-vivo plant imaging. This paper reports preliminary results regarding the technical issues associated with performing label-free imaging in plant tissues with CRS and discusses how they may be mitigated in future applications.
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