Abstract
Coherent Raman scattering (CRS) microscopies are noninvasive imaging modalities that provide label-free, chemical-specific, depth-resolved information of tissue with subcellular image resolution. CRS has been applied in various dermatological applications and has provided helpful insights for clinical diagnosis as well as basic laboratory research. CRS microscopy employs two ultrafast (picosecond or femtosecond) pulsed laser beams, and the frequency difference between the two beams is tuned to target different molecules or chemical components. This is unique in the sense that CRS can provide not only the morphological/structural information of the skin, but also the chemical and molecular information. This feature has enabled clinical dermatological diagnosis, skin disease evaluation, and drug delivery monitoring, as well as the evaluation of therapeutic effects. In addition to the basic principles of CRS techniques, this chapter will also discuss the broad applications of CRS in the field of dermatological imaging, both in basic research and clinical settings.
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