Abstract
BackgroundThe primary cell wall of fruits and vegetables is a structure mainly composed of polysaccharides (pectins, hemicelluloses, cellulose). Polysaccharides are assembled into a network and linked together. It is thought that the percentage of components and of plant cell wall has an important influence on mechanical properties of fruits and vegetables.ResultsIn this study the Raman microspectroscopy technique was introduced to the visualization of the distribution of polysaccharides in cell wall of fruit. The methodology of the sample preparation, the measurement using Raman microscope and multivariate image analysis are discussed. Single band imaging (for preliminary analysis) and multivariate image analysis methods (principal component analysis and multivariate curve resolution) were used for the identification and localization of the components in the primary cell wall.ConclusionsRaman microspectroscopy supported by multivariate image analysis methods is useful in distinguishing cellulose and pectins in the cell wall in tomatoes. It presents how the localization of biopolymers was possible with minimally prepared samples.
Highlights
The primary cell wall of fruits and vegetables is a structure mainly composed of polysaccharides
The primary cell wall of fruits and vegetables is a heterogonous structure mainly composed of polysaccharides, including a wide variety of pectins and hemicelluloses as well as cellulose
It may be that there are some particular Raman shifts which are unique to each polysaccharide and which could be used for the identification of these compounds in the cell wall material
Summary
The primary cell wall of fruits and vegetables is a structure mainly composed of polysaccharides (pectins, hemicelluloses, cellulose). It is thought that the percentage of components and of plant cell wall has an important influence on mechanical properties of fruits and vegetables. The primary cell wall of fruits and vegetables is a heterogonous structure mainly composed of polysaccharides, including a wide variety of pectins and hemicelluloses as well as cellulose. The cell wall is a composite of cellulose, hemicellulose and pectin, with the addition of other, non-polysaccharide components like proteins, lipids, enzymes and aromatic compounds. The primary cell wall is composed of approximately 25% cellulose, 25% hemicellulose and 35% pectins, with up to 8% structural proteins (on a dry-weight basis). Large deviations from these values may be found [5]
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