Abstract
Lambda phage DNA and DPPC thin films are imaged in liquids by atomic force microscopyapplying the amplitude modulation mode (‘tapping mode’) with active enhancement of theQ-factorby a ‘Q-control’ electronics. The topography of the resulting images is compared with images obtained withoutactive Q-control. To enable a meaningful comparison, individual scan lines are alternately recorded with andwithout Q-factor enhancement using scan parameters optimized for each mode separately.As the major finding, significant height differences of topographical featuresare observed between the two modes. The heights measured with activeQ-control are reproducibly higher compared to the ones observed withoutQ enhancement. This effect is attributed to the reduction of tip–sample forces byQ-control.
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