Abstract

Drug addiction is a pressing issue in modern American society that demands thorough research into its mechanisms. Cocaine locomotor sensitization is a well-established model for understanding the physiological effects of cocaine abuse. Previous studies have established loss of dopamine D2 autoreceptors in striatal tissue as a consequence of locomotor sensitization, yet how these differences in autoreceptor expression affect dopamine signaling remains unclear. Herein, we utilized non-genetically encoded near-infrared fluorescent nanosensors based on carbon nanotubes to image dopamine modulation in live acute coronal brain slices. By leveraging dopamine D2 receptor agonists along with the dopamine nanosensor, we developed a method to identify changes in density of functional D2 autoreceptors and dopamine signaling aberrations that accompany addiction, at the level of individual dopamine synapses. We apply the method to compare changes in D2 autoreceptor activity in the Nucleus Accumbens core in cocaine sensitized and saline (control) mice after prolonged cocaine exposure (5 days). Our preliminarily data indicates the decrease in the number of D2 autoreceptor in cocaine sensitized mice, consistent with the previous studies, and suggests that it leads to higher level of dopamine signaling. Furthermore, we confirm dopamine signaling changes by application of D2 Agonists (quinpirole) are from loss of individual dopamine release sites rather than changes in the quantity of dopamine released, or changes in dopamine reuptake kinetics. Our study provides a route to understand the changes in neuromodulation that underlie the neurobiology of addiction and other dopamine-centric neurological conditions.

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