Abstract
This work aimed to explore the effects of image mosaic algorithm- (IMA-) based low-dose X-rays on deoxyribonucleic acid (DNA) damage of peripheral blood lymphocytes in cardiovascular patients. 84 patients with interventional cardiovascular surgeries were selected as the research objects and divided into coronary intervention surgery (A) group, coronary angiography (B) group, radiofrequency ablation (C) group, and permanent cardiac pacemaker implantation (D) group. They were treated with IMA-based low-dose X-rays, DNA damage indicators of lymphocytes were detected, and the relationship was analyzed between those indicators and radiation dose. Results showed that the cumulative dose (CD) and dose area product (DAP) of group A were the lowest, and those of group D were obviously higher than those of the other three groups ( P < 0.01 ). Besides, the results also showed that the values of CD and DAP of patients from group A and group D were 0.38 ± 0.08 (Gy) and 0.38 ± 0.08 (Gy/cm2) and 1.37 ± 0.21 (Gy) and 1.37 ± 0.21(Gy/cm2), respectively. The comet tail DNA content (TDNA) and tail moment (TM) of each group after surgery increased dramatically compared with before surgery ( P < 0.01 ), and the TDNA and TM of group D were greatly higher than those of the other three groups ( P < 0.01 ). Therefore, TDNA and TM were markedly positively correlated with DAP and CD ( P < 0.01 ). IMA-based X-ray radiotherapy caused reliable DNA damage to peripheral blood lymphocytes of cardiovascular patients, and the greater the radiation dose, the more serious the DNA damage.
Highlights
With the acceleration of the aging process and the improvement of living standards, the incidence of cardiovascular diseases continues to increase
About 0.2 mL of the patient’s peripheral blood was extracted and processed with anticoagulation by heparin. e precooled lymphocyte separation solution was added into the centrifuge tube, and the peripheral blood was added slowly along the inner wall of the centrifuge tube. en, it was centrifuged at 3,500 rpm for 2 minutes. e off-white lymphocyte layer was transferred to a 2 mL centrifuge tube, and the phosphate buffer saline (PBS) was added into the tube
Low-Dose X-Ray Image Quality Evaluation Based on Image Mosaic Algorithm. e information entropy and absolute mean brightness error (AMBE) evaluation indicators were applied to evaluate the image mosaic quality. e original image was set as x (i, j), and the processed image was set as y (i, j); both of them had LM × N gray levels, so the gray distribution density of the processed image could be expressed as follows: ρ(x) ρ{L x}, x ∈ L
Summary
With the acceleration of the aging process and the improvement of living standards, the incidence of cardiovascular diseases continues to increase. Interventional surgery is generally performed under the guidance of X-ray fluoroscopy, and exposure is required for at least 5 minutes, usually about half an hour. During this process, both the physician and the patient will be damaged by high-dose ionizing radiation. Patients do not have any protective measures, so the radiation exposure to patient is more harmful. X-ray is a low linear energy transfer ray, which is less harmful to the human body compared with other medical rays [4, 5]. Various organs and tissues in the human body have different sensitivities to ionizing radiation. DNA is the main target molecule of the radiation effect, and the application of DNA damage as a biological dose evaluation indicator can more accurately evaluate the random effects of radiation
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