Abstract
Objective To investigate the role of interleukin-22 (IL-22)-regulated autophagy in hydrogen peroxide (H2O2)-induced hepatocarcinoma cell damage. Methods HepG2 cells were transfected with pEGFP-LC3 and then cultured in RPMI 1640 medium free of fetal bovine serum (FBS) or containing 1% or 10% FBS. These cells were pretreated with rapamycin or an autophagy inhibitor (3-MA) and then stimulated with recombinat human IL-22 (rhIL-22). GFP-LC3 puncta formation and autophagy signaling activation were measured. MTT assay was performed to detect cell viability. Results rhIL-22 significantly promoted GFP-LC3 puncta formation and LC3-Ⅱ expression in HepG2 cells treated with different stimulation protocols. The autophagy pathway inhibitor, 3-MA, dramatically suppressed the rhIL-22-activated autophagy signals. rhIL-22 attenuated H2O2-mediated HepG2 cell death and that could be inhibited by 3-MA. Conclusion IL-22 promoted the activation of autophagy signaling pathways and alleviated H2O2-mediated HepG2 cell damage. Key words: Interleukin-22; Autophagy; Hepatocarcinoma cell; Cell death; Hydrogen peroxide
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