Abstract
The aim of this research was to investigate the possible association between gastric carcinoma (GC) and polymorphisms of the IL-1β gene in the Kashmiri population using peripheral blood DNA from 150 gastric carcinoma cases and 250 population controls with detailed data for clinicopathological characteristics of the disease. Two SNPs in the IL-1β gene were selected for this study. Expression of IL-1β was studied in 50 gastric carcinoma cases using immunohistochemistry and RT-PCR and then correlated with genotype. The frequency of the IL-1β-511 C allele was significantly higher in the GC case group (53.3%) than in controls (45.4%) with an odds ratio (OR) of 0.73 and a P value of 0.03. Multivariate regression analysis showed associations of gastric carcinoma with mutant form of IL-1β-511 TT (OR 0.309; P value <0.001) and the CC genotype of IL-1β-31 (OR 0.313; P value of 0.002). Haplotype analysis of IL-1β-31 and IL-1β-511 showed decreased association of IL- 1β-31 T with IL-1β-511 C with gastric carcinoma (OR 0.728; P value 0.03). Expression study of 50 samples by immunohistochemistry (IHC) and RT-PCR showed association with grade III and stage III+IV. After correlating the expression with polymorphism no association was found.
Highlights
Gastric carcinoma is the fourth most common cancer in the world and the second leading cause of cancer death
The aim of this research was to investigate the possible association between gastric carcinoma (GC) and polymorphisms of the IL-1β gene in the Kashmiri population using peripheral blood DNA from 150 gastric carcinoma cases and 250 population controls with detailed data for clinicopathological characteristics of the disease
The frequency of the IL-1β-511 C allele was significantly higher in the GC case group (53.3%) than in controls (45.4%) with an odds ratio (OR) of 0.73 and a P value of 0.03
Summary
Gastric carcinoma is the fourth most common cancer in the world and the second leading cause of cancer death. These SNPs may affect the overall expression and secretion of cytokines and may account for some of the heterogeneity of infectious diseases These two SNPs were associated with an increased risk of gastric carcinoma in Scottish and Polish subjects (El-Omar et al, 2000) and were subsequently confirmed by studies in other ethnic groups from the USA (El-Omar et al, 2003) and Portugal (Machado et al, 2003). IL-1β Polymorphisms and Risk of Gastric Carcinoma: a Case Control Study in an Ethnic Kashmiri Population scores. A total of 150 gastric carcinoma patients and 250 first-strand cDNA as a template, the specific primers for control subjects were included in this study. GAPDH acted as control for sample to sample variations and association of CC genotype of IL-1β-31 with odds in reverse transcription and PCR conditions and to control ratio of 0.313 and P value of 0.002 was found. Statistics for haplotype analysis (Table 4) of IL-1β-31 and IL-1β-511 shows association of IL-1β-31 T with
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