IL-17A differential effects in murine bone marrow granulopoiesis in presence of GM-CSF or IL-5 in vitro

Abstract

Event Abstract Back to Event IL-17A differential effects in murine bone marrow granulopoiesis in presence of GM-CSF or IL-5 in vitro Lysianne Pinto1*, Tulio Queto1, 2, Daniela Brito1, Cassio Coutinho1, Bianca De Luca2, Bruno Vieira1, 2, Jose Carlos Alves Pinto3, Fernando Cunha3, Maria Ignez Gaspar-Elsas2* and Pedro Xavier-Elsas1* 1 Universidade Federal do Rio de Janeiro, Immunology, Brazil 2 FIOCRUZ, Brazil 3 USP-FMRP, Brazil Interleukin (IL)-17A plays diverse roles in autoimmunity and allergy. Its best known functions are stimulatory and selective for the neutrophil lineage. However, IL-17A effects on granulopoiesis remain in completely understood. We examined IL-17A effects on eosinophil and neutrophil production from cultured murine bone-marrow (BM). In IL-5-stimulated liquid cultures, IL-17A suppressesd eosinophilopoiesis. by contrast, IL-17A enhanced GM-CSF-stimulated eosinophilopoiesis. Downregulation of IL-5-stimulated eosinophilopoiesis by IL-17A was prevented by addition of zVAD-fmk (a terminal caspase inhibitor) and by aminoguanidine (a selective iNOS inhibitor). In cultured BM iNOS- or IL-17AR-deficient mice, suppression of IL-5-stimulation eosinophilopoiesis was undetectable, contrasting with wild type controls. In wild-type BM (C57BL/6 or BALB/c), synergism was detectable when IL-17A and sodium nitroprusside (a NO donor) were present together, whereas either agent, added separately to the culture, had no effect on BM from both iNOS-deficient and wild-type donors. By contrast, the upregulation of eosinophilopoiesis in wild-type GM-CSF-stimulated BM cultures by IL-17A was iNOS-independent. In progenitor (clonal) semi-solid cultures seeded with GM-CSF, colony formation was decreased by 2 ng/ml (but not by 0.2 ng/ml) IL-17 A. Together, these results suggest divergent mechanisms of action for IL-17A as a regulator of BM granulopoiesis, depending on the cytokine environment, which may reflect the involvement of different subsets of target cells in hemopoietic tissue, which are broader for GM-CSF (multilineage stimulus) than for IL-5 (eosinophil-selective stimulus), at different steps. Acknowledgements CNPq FAPERJ FIOCRUZ Keywords: Granulopoiesis, IL-17A, IL-5, i-nos, Apoptosis Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Pinto L, Queto T, Brito D, Coutinho C, De Luca B, Vieira B, Alves Pinto J, Cunha F, Gaspar-Elsas M and Xavier-Elsas P (2013). IL-17A differential effects in murine bone marrow granulopoiesis in presence of GM-CSF or IL-5 in vitro. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00195 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 10 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Lysianne Pinto, Universidade Federal do Rio de Janeiro, Immunology, Rio de Janeiro, Brazil, lysianne@uol.com.br Dr. Maria Ignez Gaspar-Elsas, FIOCRUZ, Rio de Janeiro, Brazil, gasparelsas@gmail.com Prof. Pedro Xavier-Elsas, Universidade Federal do Rio de Janeiro, Immunology, Rio de Janeiro, Brazil, pxelsas@yahoo.com.br Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Lysianne Pinto Tulio Queto Daniela Brito Cassio Coutinho Bianca De Luca Bruno Vieira Jose Carlos Alves Pinto Fernando Cunha Maria Ignez Gaspar-Elsas Pedro Xavier-Elsas Google Lysianne Pinto Tulio Queto Daniela Brito Cassio Coutinho Bianca De Luca Bruno Vieira Jose Carlos Alves Pinto Fernando Cunha Maria Ignez Gaspar-Elsas Pedro Xavier-Elsas Google Scholar Lysianne Pinto Tulio Queto Daniela Brito Cassio Coutinho Bianca De Luca Bruno Vieira Jose Carlos Alves Pinto Fernando Cunha Maria Ignez Gaspar-Elsas Pedro Xavier-Elsas PubMed Lysianne Pinto Tulio Queto Daniela Brito Cassio Coutinho Bianca De Luca Bruno Vieira Jose Carlos Alves Pinto Fernando Cunha Maria Ignez Gaspar-Elsas Pedro Xavier-Elsas Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.