III. Effect of Dyes on Colonies of Certain Pathogenic Fungi.

Abstract

Observations were made on growth and coloring of colonies of certain pathogenic fungi cultured on a medium (4% peptone, 1% dextrose, 1 1/2% agar, pH 5.6) containing alcoholic nigrosine, litmus, eosin Y and eosin B, respectively., In the work here reported 5 batches of a similar medium containing the following percentages of dyes respectively, 2% fluorescein, 1% methyl blue and 1% eosin Y, 1/2% neutral red, 1/2% janus green, 1/2% Wright's stain Suspension, were studied. The organisms were grown in diffused light at room temperature and observed over a period of 45 days. The following pathogenic fungi and 2 non-pathogenic saprophytes, Lichthiemia sp. and Scopulariopsis brevicaulis, were observed: Achorion sckoenleinii, Acladium castellcmi, Candida candida, Endodermophyton tropicale, Endomyces capsulatum, Endomyces dermatitidus, Epidermophyton cruris, Epidermophyton inguinale, Glenospora gammeli, Geotricheum bachmann, Indiella americama, Microsporon audouini, Microsporon felineum, Microsporon gypseum, Monilia albicans, Monosporum apiospermum, Oöspora humi, Sporotrichum schenkii, Trichophyton crateriforme, Trichophyton granulosum, Trichophyton gypseum lacticolor, Trichophyton interdigitale, Trichophyton niveum, Trichophyton sulfureum, Willia anomala. Table I gives the time of appearance of growth and its relative amount. The following abbreviations are used: F, fluorescein; MB & E, methyl blue and eosin Y; Wr., Wright's stain Suspension; NR neutral red; JG janus green; b, blue; br, brown; c, copper colored; g, golden; p, pink; r, red; s, salmon; sc, scarlet; t, tinted; v, varies; y, yellow; y— (letter followed by dash) indicates color centrally; —y— (dash letter dash) indicates color between center and border.