Abstract
In the present study, patients with acute OROV fever were classified as early seroconverters (IgM/IgG positive at baseline) or late seroconverters (IgM/IgG negative at baseline) and the timeline kinetics of the production of chemokines and cytokines were assessed at 1–3, 4–7, 8–10 and ≥11 days after patients have reported the first symptoms. Regardless immunoglobulin profile, all OROV fever patients presented higher levels of CXCL8, and IFN-α and lower levels of TNF and IL-10 at baseline as compared to healthy donors (HD). Lower levels of CCL2, CXCL10, and IFN-γ and higher levels of CCL2, CXCL10, IL-6, and IL-17A were detected in early and late seroconverters, respectively, as compared to HD. While early seroconverters presented the increasing levels of CCL2 along the timeline, late seroconverters displayed decreasing levels of CCL2, CXCL10, and IL-6 following days of disease onset. Noteworthy was that IFN-α was revealed as universal biomarker of human OROV fever, while CXCL8 & IL-5 and CXCL10 & IL-17 were consistently observed in early and late seroconverters, respectively. Thus, our results suggest that the production of IFN-α, CXCL10, and IL-17 precede the seroconversion bringing novel insights on the immunological events triggered by the OROV disease.
Highlights
In the present study, patients with acute OROV fever were classified as early seroconverters (IgM/IgG positive at baseline) or late seroconverters (IgM/IgG negative at baseline) and the timeline kinetics of the production of chemokines and cytokines were assessed at [1,2,3, 4,5,6,7, 8,9,10] and ≥11 days after patients have reported the first symptoms
In order to identify the profile of circulating IgM/IgG anti-OROV in patients with acute infection, serum samples from patients at different days upon disease onset were evaluated by MAC ELISA
IgM/ IgG titers in early seroconverters were higher than late seroconverters at [1,2,3] and [4,5,6,7] subgroups
Summary
Patients with acute OROV fever were classified as early seroconverters (IgM/IgG positive at baseline) or late seroconverters (IgM/IgG negative at baseline) and the timeline kinetics of the production of chemokines and cytokines were assessed at [1,2,3, 4,5,6,7, 8,9,10] and ≥11 days after patients have reported the first symptoms. Our results suggest that the production of IFN-α, CXCL10, and IL-17 precede the seroconversion bringing novel insights on the immunological events triggered by the OROV disease. The present study aimed at investigating soluble immunological biomarkers including circulating cytokines and chemokines in early and late seroconverters along days after OROV fever onset. The results show conspicuous differences in the cytokine and chemokine levels between these two groups, which suggest that the innate immune response pattern harnessed by OROV is closely associated to seroconversion
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