Abstract
Monoclonal nonspecific suppressor factor (MNSF) is a lymphokine derived from a murine T cell hybridoma. The action of MNSF is mediated by specific cell-surface receptors. Since IFN-γ alters the cellular response to MNSF (M. Nakamura, H. Ogawa, and T. Tsunematsu, J. Immunol. 138, 1799, 1987), we investigated whether IFN-γ has an effect on the expression of MNSF receptor on target cells. IFN-γ enhanced the expression of MNSF receptor on both MOPC-31C cells (a murine plasmacytoma line) and EL4 (a murine T lymphoma line). Incubation with IFN-γ increased the number of specific MNSF-binding sites by about 50 to 90%, with no significant change in binding affinity. IFN-α and IFN-β also increased MNSF binding, although the effect of the saturating amounts was lower than that seen with IFN-γ. Maximal enhancement of receptor expression was observed after about 15 hr of incubation with IFN-γ. No demonstrable change occurred in the kinetics of internalization of 125I-MNSF bound to MOPC-31C cells preincubated without or with IFN-γ.
Published Version
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