Identifizierung verschiedener Miscanthus‐Sorten mittels Isoenzymen

Abstract

AbstractIdentification of Miscanthus Varieties by their IsozymesWith the aim of testing the possibility of identifying Miscanthus varieties, zymograms of 13 isozymes from 65 plants of M. smensis, M.×giganteus and M. sacchariflorus were analysed using starch‐gel electrophoresis. The zymograms of root, rhizome, and leaf tissues were compared. After similarity between the zymograms of the different types of tissues had been established, the tissue of young leaves was used for analysis. The zymograms of the different isozymes are variable in most cases and show as many as nine patterns. The large variation between the varieties allows an identification of each of the varieties analysed. About half of the plants could be attributed to M.×giganteus. In this group of plants, different variants were found in the isozymes of diaphorases and glutamate‐oxalacetate‐transammases. These variants might indicate that M.×giganteus includes more than one genotype. Some of the plants which were assumed to belong to M.×giganteus could clearly be excluded from this variety. Although some of the varieties can only be unequivocally identified by their flowers, it is possible to analyse the isozymes even in the dormant state of the rhizomes, thus, this method offers a simple way of identifymg Miscanthus varieties by the principle of exclusion, independent of the physiological state of the plant.