Abstract
Sweet Flag (Acorus calamus L.) leaf and rhizome tissues were analyzed for biochemical compositions notably of carbohydrates and lipids. The glycolipid content measured in rhizome tissue was 62.3 mg%/FW almost double the glycolipid content (28.8 mg%/FW) in leaf tissues, whereas the sterol content in the leaf tissue (47.9 mg%/FW) was three times of the sterol content in rhizome tissues (15.5 mg%/FW). Carbohydrates content such as total sugar, reducing sugar, sucrose and fructose measured in leaf and rhizome tissues were more or less similar, with slightly higher values of total sugar (18.2 mg%/FW) in the leaf tissues. The study thus revealed variation in biochemical compositions in two different tissues leaf and rhizome of A. calamus.
Highlights
Total lipids were extracted according to the Folch's method.[11]
Methanol was added to make the ratio 1:1.The crude lipid extract was transferred into a separating funnel and 1% NaCl solution was added to one forth of its volume
Sucrosyl fructose was determined by dividing studies have reported phyto-chemical composi- Glycolipids were estimated in the crude lipid the concentration of sucrose by 1.9 and the tions of A. calamus whole plant and its differ- extract as previously published procedures.[12,13] non-sucrosyl fructose from the difference in ent parts viz., roots, rhizomes, leave and essen- Sterols and free fatty acids in the lipid extracts values of total and sucrosyl fructose. tial oil.[7,8,9]
Summary
Total lipids were extracted according to the Folch's method.[11]. Leaves and rhizomes were weighed (10 g), made in to small pieces and mixed with 30 ml of chloroform: methanol (2:1 v/v) and kept overnight at room temperature for extraction of the lipids. The sugar free residue was used to estimate starch content.
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