Abstract
The chemical compound of CO gas is a gas that has no color and contributes greatly to environmental pollution as a result of incomplete combustion of fuel produced from motor vehicles. Carbon monoxide is very dangerous (toxic, so it is often referred to as the "silent killer". The presence of CO gas will be very dangerous if inhaled by humans because the gas will replace the position of oxygen that binds to hemoglobin in the blood. The purpose of the study was to identify the presence or absence of carbon monoxide (CO) in the blood of fruit sellers at the Sungguminasa Twin Bridge. This research is an analytical observational field research using the alkaline dilution test method. The sampling technique used purposive sampling with the criteria of working more than one year, working 8 hours a day and not smoking. The number of samples used as many as 9 samples of venous blood. From the results of this study, it can be concluded that the presence of carbon monoxide gas was not identified in all blood samples of fruit sellers at the Sungguminasa Twin Bridge. This is because in the alkaline dilution test method, CO gas can only be identified with saturation levels above 20%.
Highlights
greatly to environmental pollution as a result of incomplete combustion of fuel produced from motor vehicles
The presence of CO gas will be very dangerous if inhaled by humans because the gas will replace the position of oxygen
that binds to hemoglobin in the blood
Summary
Bahan yang digunakan adalah Sampel darah, larutan NaOH 10% dan aquades. Diarahkan pasien mengepal tangannya lalu dilakukan pengambilan darah dengan menusuk vena menggunakan jarum dengan sudut 15-30 derajat. Lalu jarum ditarik keluar perlahan dengan memberikan kapas kering pada bagian yang telah ditusuk. Prosedur Kerja Uji Alkali Dilusi adalah sebagai berikut: Tabung reaksi diisi masing-masing 10 ml aquadest. Lalu ditambahkan 5 tetes NaOH 10% pada masing-masing tabung reaksi lalu dihomogenkan hingga darah kontrol berubah warna menjadi hijau kecoklatan (Linda, A.V, et al 2016). Interpretasi Hasil adalah sebagai berikut: Darah normal akan segera berubah warna menjadi hijau kecoklatan ketika ditetesi NaOH 10% karena terbentuk hematin alkali. Sedangkan darah yang positif mengandung CO dengan kadar saturasi 20% tidak berubah warnanya dalam kurun waktu satu menit, dengan mempertahankan warna pertama (merah muda) tergantung pada konsentrasi CO karena bersifat lebih resisten terhadap pengaruh alkali. Data hasil uji kontrol dan hasil penelitian disajikan pada tabel 1 dan 2
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