Abstract
This research aims to identify the Staphylococcus aureus (S. aureus) bacteria from preputium and vagina of horses. Five swabs preputium and 5 swabs vagina samples from 3-9 years olds horse in the village of Bebangka, Gelelungi, Reloop, and Kelapeng, Pegasing Aceh Tengah District were used in this study. Preputium and vagina swabs of horses were dipped into reaction tube containing Nutrient Broth (NB). Staphylococcus aureus was identified on manitol salt agar (MSA). Separated colony colored bright yellow on the media were tested for Gram staining, hemolysis, catalase, and sugar test (glucose and manitol). The result showed that the isolates growth on MSA were colored bright yellow, Gram positive, β hemolisis, and catalase positive. The isolates fermented manitol and glucose. Isolates identified from horses preputium and vagina showed the characteristic of S. aureus. In conclusion S. aureus bacteria were positively identified from all of swabs samples, both in preputium and vagina of horses.Key words: Staphylococcus aureus, horse, preputium, vagina
Highlights
Populasi kuda secara keseluruhan di Indonesia adalah 454.340 ekor dan di Aceh populasi kudanya dari tahun 2009-2013 berturut-turut adalah 3.362; 3.463; 2.495; 2.314; 2.331 (Direktorat Jenderal Peternakan, 2009-2013)
5 swabs vagina samples from 3-9 years olds horse in the village of Bebangka
Pegasing Aceh Tengah District were used in this study
Summary
Yulina Rahmi, dkk kerbau lumpur (Swamp buffalo) di Aceh Barat Daya seluruhnya positif teridentifikasi terinfeksi S. aureus. Pengujian ilmiah juga dilakukan oleh Fardi (2012), membuktikan dari 5 swab preputium kerbau (Babalus bubalis) di Aceh Barat Daya ditemukan lima jenis genus bakteri Gram negatif yaitu E. coli, Salmonella sp., Citrobacter sp., Klebsiella sp., dan Proteus sp. Penelitian yang dilakukan oleh Gunay et al (2010), menunjukkan swab vagina pada 55 ekor anjing betina yang mengalami tahap siklus estrus tidak ada teridentifikasi bakteri S. aures, jenis bakteri yang teridentifikasi seperti, E. coli, Proteus sp., Pseudomonas sp., Streptococcus sp., dan Enterococcus sp
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