Identification study for suspected women with Trichomonas vaginalis by PCR technique in AL-Najaf AL-Ashraf province

Abstract

The study was conducted on 450 out patients and 30 healthy women, whom have visited the department of infertility at Al-Sadder medical city and Al-Zahra Hospital in Najaf Province during the period from January till August, 2012. The infection with T.vaginalis in clinical suspected women determine by using PCR technique, the infection women numbers and percent by PCR test gave 58 and 12.88% respectively. DNA of T.vaginalis parasite was determined by PCR was using five specific primers (BTUB 9/2, BGL 1/2, TVA 5/6, TV 1/2 and OP 1/2) whereas the number, percent and sensitivity of infected women of these primers were 58 (12.88%) (100%), 56 (12.44%) (96.55%), 53 (11.77%) (91.37%) and 52 (11.55%) (89.65%) respectively, from comparing between these primers to evaluate the efficiency in diagnostic of T.vaginalis .Indicated that BTUB 9/2, BGL 1/2 the highest primers and OP 1/2 the lowest primers in diagnostic efficiency.