IDENTIFICATION OF YEASTS SPECIES COLLECTED FROM PATIENTS USING OF MALDI -TOF MASS SPECTROMETRY TECHNIQUE AND SEQUENCING

Abstract

Background: Yeasts are important opportunistic pathogen in human, in which Candida spp. are the most common causative agents. This study was carried out on 121 yeast strains collected from 103 patients in Hue University Hospital and Hue Central Hospital from January 2013 to June 2014. Objective: To identify yeasts species from systemic mycoses and superficial mycoses. Methods: We applied MALDI - TOF Mass Spectrometry techniques, PCR and DNA sequencing to detect yeasts species. Results: There were 121 yeast strains collected, in which C.albicans 43.80%, C.tropicalis 17.36%, C.parapsilosis 11.75%, C.glabrata 7.44%, C.orthopsilosis 4.96%, C.metapsilosis 0.83%, C.krusei 3.31%, C.norvegensis 0.83%, C.guilliermondii 0.83%, C.digboiensis 2.48%, C.famata 1.65%, C.blankii 0.83%, C.mesorugosa 0.83%, Geotrichum capitatum 1.65%, Trichosporon asahii 1.65%. Conclusions: In our study, the prevalences of C.abicans, C.non albicans and other yeasts species were 43.80%, 47.90% and 3.30% respectively. We reported some rare species of Candida non albicans, including C. orthopsilosis, C.metapsilosis, C.norvegensis, C.digboiensis, C.blankii, and C.mesorugosa. Key words: Yeast, Candida spp., MALDI - TOF Mass Spectrometry