Abstract
AbstractPavlova gyrans andP. lutheri were cultured, and the dihydroxysterols were isolated from the free sterol and the polar sterol fractions. Four dihydroxysterols were detected in amounts greater than 1% of total sterol by gas chromatography and were analyzed by gas chromatography/mass spectrometry. The two principal sterols were isolated by chromatography on alumina followed by hydrophobic Sephadex column chromatography. The two sterols appeared to differ by having either a methyl or an ethyl group at C−24; they were termed “methylpavlovol” and “ethylpavlovol.” Analysis by 400 MHz nuclear magnetic resonance showed that methylpavlovol is 4α,24β‐dimethylcholestan‐3β,4β‐diol and ethylpavlovol is 4α‐methyl,24β‐ethylcholestan‐3β,4β‐diol. The 4α‐methyl,4β‐hydroxy configuration has not been observed previously in a natural sterol. Dihydroxysterols make up approximately one‐third of the total sterols in thesePavlova species. Neither the biosynthetic origin of these dihydroxysterols nor their role in the biochemistry ofPavlova is known.
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