Abstract
This paper reports multilocus electrophoresis for identification of Tuber ectomycorrhizae. The commercial value of some species (e.g., T. magnatum, T. melanosporum and T. aestivum) makes their identification particularly important at the ectomycorrhizal stage, for inoculating practices as well as to track the environmental fate of the inoculated species. The electrophoretic ectomycorrhizal patterns at 4 enzymatic loci, obtained by modifying methods of enzymatic extraction as well as some staining procedures, allowed the identification of wild and nurseryinoculated ectomycorrhizal samples of Tuber spp. The results obtained indicate that the electrophoretic analysis of gene-enzyme systems is a reliable alternative method to the conventional morphometric criteria of fungal identification. At the same time, it is an useful tool for a rapid and simple identification of truffles during the ectomycorrhizal stage for large scale controlled production.
Published Version
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