Identification of the potential mechanisms of Huzhang Tongfeng Granules against acute gouty arthritis through network pharmacology and in vivo analysis

Abstract

ObjectiveTo identify the potential mechanism of Huzhang Tongfeng Granules (HTG) for the treatment of acute gouty arthritis (AGA). MethodsFirst of all, we used network pharmacology to build a network of components-targets of HTG. Secondly, the ClusterONE algorithm was applied to construct a modular network as well as discover hub genes and kernel pathways. Lastly, the MSU crystals-induced AGA mice model was used to verify the hub genes and kernel pathways of HTG. ResultsHTG down-regulated the expressions of ADRB3, PIK3CA, FYN, PIK3R1, CASR, PLCG1, SYK, EGFR, and up-regulated MC1R, MC5R. Moreover, according to the kernel clusters enrichment analysis, HTG down-regulated NF-κB and P13K-Akt signaling pathways to ameliorate the AGA mice model. ConclusionIn summary, the potential targets and kernel pathways were proved in this study, supporting the clinical application of HTG for the treatment of AGA.