Identification of the antibacterial action mechanism of curcumin on Streptococcus mutans through transcriptome profiling

Abstract

ObjectiveThe purpose of this study was to explore the effect and mechanism responsible for how curcumin affects the biofilm formation by Streptococcus mutans (S. mutans). DesignThe antibacterial activity of curcumin was evaluated by measuring the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). The mass of the biofilm was measured by crystal violet staining. Transcriptome sequencing was used to obtain all the transcript information associated with the biological activity of curcumin-treated S. mutans. Real-time quantitative PCR (qRT-PCR) was performed to examine the expression levels of related biofilm formation genes. ResultsThe MIC value for curcumin was 64 μM. Curcumin inhibited the formation of a biofilm by S. mutans and degraded mature biofilms. A gene ontology enrichment analysis showed that the DEGs were significantly relevant to biofilm formation. In addition, 17 significantly enriched Kyoto Encyclopedia of Genes and Genomes pathways (p ≤ 0.01) were identified and were potentially associated with the biochemical metabolic processes of S. mutans. DEGs associated with the biofilm formation of S. mutants, including gtfB, gtfC, rgpG, spaP, spxA1, spxA2, bacA, lrgB, and gshAB. The qRT-PCR results were consistent with transcriptome sequencing that the expression levels of gtfB, gtfC, rgpG, and spaP significantly decreased in the curcumin-treated group, whereas the expression levels of spx1, spx2, bacA, lrgB, and gshAB were up-regulated. ConclusionsCurcumin showed marked inhibitory effects against the formation of biofilms by S. mutans and degradation of formed biofilms.