Abstract
Objective To identify tapeworm species in Dali,Yunnan Province.Methods After morphological identitlcation of worms in Dali,genomic DNA of Taenia cestode isolate was extracted from segments of adult tapeworms.partial segments of mitochondrial DNA-cytochrome B(mtDNA-Cytb)sequences were Rillplified by PCR and then sequenced.Phylogenetic tree of species wag constructed with other known mtDNA Cytb of T.solium,T.saginata and T.asiatica in GenBank using DNAMAN software.Results The Dali(E1)isolate was similar to T.saginata with morphological observation.The phylogenetic tree constructed revealed that the Taenia cestode isolate samples(E1,E2,SL,WS)in Dali,Yunnan Province were close to T.asiatica.but far from T.saginata,even farther from T.solium genetically.Hl,H2 isolates were close to T.soli um and far from T.saginata and T.asiatica.Conclusion Morphological and molocular classification showed that Dali(E1,E2,SL,WS)isolates belong to T.asiatica while H1,H2 belong to T.solium. Key words: Taenia saginata; Taenia solium; Taenia asiatica; Molecular classification; Morphology
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