Identification of strains and screening of resistance resources to soybean mosaic virus in Anhui Province

Abstract

In order to clarify the local constitution,distribution,and population dynamics of soybean mosaic virus( SMV) in Anhui Province and to screen resistant accessions to epidemic SMV strains,461 potential SMV samples of soybean leaves have been collected from main soybean regions in Anhui Province during 2009-2010. Of those,83 SMV isolates were identified through expanding propagation,single lesion isolation purification,serological determination. They were grouped into the 14 SMV strains including SC3-SC9,SC11,SC13-SC15,SC17,SC19 and SC22,based on their response to 10 unified national soybean differentials. Of the 14 SMV strains,7strains were found for the first time in this study,encompassing SC5,SC11,SC14,SC15,SC17,SC19 and SC22.The comparison between present and previous identification results of SMV strains showed that SC3 and SC7 strains were still predominant in Anhui Province with 16. 9% and 25. 3% of the total number of isolates,and SC15 was lower with 12. 1%. In terms of soybean accessions resistant to popular strains of SC3 and SC7,5 of 149 accessions,Zhongzuo J8023,Zhonghuang 45,L85-2308,Zhongguo 47 and Suike 13 showed high resistance to SC3strain and 9 accessions to SC7,including Zhonghuang 45,Su03-4-1-4 and Lu06-7 etc. 13 accessions displayed resistant to SC15,account for 13. 7% of the 95 tested soybean accessions. In this paper,we clarified the SMV predominant strains and their variation trend in the main soybean producing regions of Anhui Province,providing the basis for the breeding and layout of resistant soybean accessions to the disease.