Identification of a novel Soybean mosaic virus isolate in China that contains a unique 5′ terminus sharing high sequence homology with Bean common mosaic virus

Abstract

Two soybean viral isolates 4469-4 and Sc6 from plants in China were characterized by serological assays, pathogenicity tests, full-genome sequencing and sequence analyses. Both isolates are determined to be Soybean mosaic virus (SMV) isolates but 4469-4 is different from other known SMVs by inducing symptoms on common bean. 4469-4 has an RNA genome of 9994 nucleic acids (nt) encoding 3202 amino acids (aa), which is approximately 400 nt longer than that of Sc6 and other SMV strains. Comparison with SMV and SMV-related potyviruses suggests that 4469-4 shares high nt and aa sequence identify (>92%) with other SMV strains. However, significant diversity between 4469-4 and other SMV strains was observed in the 5′ genomic region. In contrast, this region is highly similar to the corresponding region of Bean common mosaic virus (BCMV). Recombination analyses conclude that there is a recombination site near the nt 900 of 4469-4. Taken together these data suggest that 4469-4 may result from recombination between SMV and BCMV or a BCMV-like virus in the N-terminus of the genome.