Abstract
Bamboo is a typical fast-growing non-timber bioresource in tropical and subtropical forests, yet its vulnerability to drought is one of the facing difficulties in commercial cultivation. The mechanism strategy in response to drought is poorly understood in bamboo plants. This study identified Sucrose non-fermenting 1-related protein kinase 2 s (SnRK2s) in the moso bamboo (Phyllostachys edulis), a group of kinases essential for plant adaptation. The expansion of PeSnKR2 family was driven by gene duplication. 18 PeSnKR2s were categorized into 3 subgroups based on their evolutionary relationships. Cis-elements analysis and expression analysis revealed that PeSnRK2s might be involved in stress resistance through ABA-dependent or ABA-independent pathways. 9 ABA-dependent and 9 ABA-independent PeSnKR2s were defined according to their sensibility to ABA. Furthermore, we examined the function of two ABA-independent SnRK2s, PeSnRK2.2A and PeSnRK2.2B, which were relevantly conserved at protein, transcript, and genomic levels, while showing distinct expression patterns under drought conditions. A transgenic rice study revealed that overexpression of either PeSnRK2.2A or PeSnRK2.2B relieved the overwhelming ROS in leaves to improve rice resistance under drought. The ABA signaling pathway was activated in transgenic rice indicating that PeSnRK2.2A or PeSnRK2.2B regulated tolerance via a conserved ABA-regulated manner downstream. Therefore, we concluded that PeSnRK2.2B and PeSnRK2.2A have similar roles in regulating ABA signals, but respond differently to environmental signals in P. edulis. PeSnRK2.2s represent the potential targets for the improvement of bamboo agronomic traits in the future. This study improves the understanding of drought-resistance mechanisms in moso bamboo and benefits genetic improvement and maintenance of bamboo sustainability.
Published Version
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