Identification of Shiga toxin producing E. Coli from raw Meat

Abdul Razzaq

doi:10.19045/bspab.2016.50033

Abstract

Emergence of Shiga toxin genes in Shiga toxin producing E. coli (STEC), all over the world, has become problematic and causes GIT illnesses in human originating in food of animals mainly from poultry. The aim of this study was to render rapid diagnostics of Shiga toxin producing E. coli (STEC) from raw meat. During a 4 month period from May to August a total of 200 samples were collected from beef (100) and chicken (100) and analyzed for Shiga toxin producing E. coli. Of the total of 200 samples of raw meat only chicken (2%) and beef (1%) were detected as PCR positive. Over the past decade many improvements have been made both in conventional and modern technique for detection of bacterial pathogens in food that include sample preparation, plating techniques, counting and identification kits but polymerase chain reaction technique is increasingly used which is considered more specific, sensitive, rapid and cost effective. Raw meat could be a source of Shiga toxin producing E. coli which indicates that possible risks of infections to people could be transferred by the consumption of raw meat and their rapid diagnostics could be made possible by the use of rapid diagnostic technique polymerase chain reaction.

Highlights

Introduction illnesses and are called diarrheagenicE. coli.In the intestinal micro flora of humans andIt harmlessly colonizes the intestine but mammals Escherichia coli (E. coli) are several strains of E. coli cause various predominantly found
Enterobacteriaceae and this bacterium is one motile [1]. It has been divided into six of the inhabitants of intestine. These pathotypes that includes atypical commensals never cause a problem in their enteropathogenic E. coli (ATEC), host but some pathogenic E. coli are enteropathogenic E. coli (EPEC), involved in diarrhea and other enteric enteroaggregative E. coli (EAEC), Published by Bolan Society for Pure and Applied Biology enterotoxigenic E. coli (ETEC), enterohemorrhagic or Shiga toxin producing E. coli (EHEC/STEC) and enteroinvasive E. coli (EIEC) [2]
Shiga toxin producing E. coli (STEC) is responsible for causing an increasing number of human outbreaks, characterized by bloody diarrhea, non-bloody diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS) and 85% of these cases are implicated with foodborne transmission. stx is further subdivided into two families’ stx1 and stx2 on the basis of sequence analysis

Summary

Introduction

Introduction illnesses and are called diarrheagenicE. coli.In the intestinal micro flora of humans andIt harmlessly colonizes the intestine but mammals Escherichia coli (E. coli) are several strains of E. coli cause various predominantly found. It has been divided into six of the inhabitants of intestine These pathotypes that includes atypical commensals never cause a problem in their enteropathogenic E. coli (ATEC), host but some pathogenic E. coli are enteropathogenic E. coli (EPEC), involved in diarrhea and other enteric enteroaggregative E. coli (EAEC), Published by Bolan Society for Pure and Applied Biology enterotoxigenic E. coli (ETEC), enterohemorrhagic or Shiga toxin producing E. coli (EHEC/STEC) and enteroinvasive E. coli (EIEC) [2]. The conventional approaches to detect food related bacteria rely on the selective enrichment and culture characteristics followed by biochemical characterization These methods are time consuming, labor intensive and often not reliable in contrast to PCR which is a rapid molecular test with high sensitivity and specificity Material and method A total of 200 samples including raw chicken (100) and beef (100) were collected in PBS from various regions of Lahore and were carried to the laboratory under refrigerated conditions. All PCR reagents were supplied by Thermo scientific USA and all PCR assays were performed in PCR Master Cycler (Eppendorfs Germany)

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