Abstract
The Pi9 gene in rice confers resistance to strains of blast pathogen Magnaporthe oryzae. Pi9 is a typical broad spectrum resistance gene containing nucleotide binding site, leucine rich repeat family of sequences. In the present study, presence of the Pi9 gene in 47 rice germplasm accessions was determined using dominant sequence tagged site marker 195R-1/195F-1 derived from the Nbs2-Pi9 candidate gene and resistance confirmed by inoculating rice germplasm with a mixture of aggressive isolates of M. oryzae namely Mo-ei-66, Mo-ei-79, Mo-ei-119, and Mo-ei-202. The Pi9 gene was found in six rice germplasm accessions from eastern India. Usefulness of this STS marker for determination of the genotype of rice germplasm was thus demonstrated. Rare occurrence of Pi9 gene in the evaluated rice germplasm suggests that its introgression is very less in indica rice. These results are useful for incorporating Pi9 gene into elite cultivars by marker assisted selection in rice breeding programs worldwide.
Published Version
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