Abstract
Abstract － One of the WHSC1/MMSET/NSD2 variant RE-IIBP is a histone H3-K27 methyltransferase with transcriptional repression activity. Overexpression of RE-IIBP in various types of leukemia suggests it’s role in leukemogenesis. Here we identify two proteins, KRAB zinc finger protein ZNF 350 and enolase-1 as RE-IIBP interacting proteins by yeast two-hybrid screening and confirmed direct interaction in vivo and in vitro . Both proteins have been known for their role in transcriptional repression. Reporter assays using transient transfection demonstrated that both ZNF 350 and enolase-1 proteins synergistically repressed transcription with RE-IIBP, respectively. These results indicate both proteins have roles in RE-IIBP mediated tran-scriptional repression by involving co-repressor complex.Keywords: RE-IIBP, Yeast two-hybrid screening, ZNF 350, Enolase-1 INTRODUCTION In all eukaryotes, the histone modification plays an im-portant role in the transcriptional regulation, development, and DNA repair (Kouzarides, 2007). The methylation of histone tails at specific residues are five lysines within K4, K9, K27, K36, K79 on H3 and one lysine K20 within histone H4 that have been methylated by specific histone methyl-transferase (HMTase) (Sims
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
