Abstract
A bacterial strain, designated AcBz01, was isolated from a water sample collected from Gomti River, Lucknow, India, and identified using a molecular approach. On the basis of the bacterial 16S rRNA gene sequence phylogeny and comparison of this gene sequence with sequences in Ribosomal Database project II, evidence given in this study, it is proposed that isolate is closely related to members of the genus Acinetobacter. Identification and annotation of regulatory elements in the 16S rRNA gene and characterization of their interaction with the respective transcription factor can provide basis for better understanding of the mechanism of network of gene interaction of functionally related genes. The identification of such sites is relevant for locating promoter boundary of a gene and it also allows the prediction of specific gene expression pattern and response to disturbances in a known signaling pathway. Computational identification of regulatory elements and Transcription Factor with their binding sites in 16S rRNA gene of Acinetobacter sp. was performed using BPROM tool. We predicted the regulatory elements are TSS, -10 box, -35 box and three Transcription Factor (narP, ompR and fadR) with their binding sites in the upstream region of 16S rRNA gene of Acinetobacter sp. AcBz01. The GenBank accession number for 16S rRNA gene of Acinetobacter sp. AcBz01 is EU931637.
Highlights
Gomti, one of the rivers in India, represents a unique niche for many animals and plants in Lucknow, which is one of the cities of India
Comparison of the bacterial 16S rRNA gene sequence has emerged as a preferred genetic technique [1]
We reported the isolation and identification of a previously unknown bacterium from Gomti, for which the name Acinetobacter sp
Summary
One of the rivers in India, represents a unique niche for many animals and plants in Lucknow, which is one of the cities of India. The regulatory elements TSS, -10 box, -35 box and known Transcription Factor (TF) with their binding sites in the upstream sequence of bacterial 16S rRNA gene of Acinetobacter sp. It is evident from phylogenetic analysis of for one minute. Discard the flow-through and centrifuge the Genomic regulatory elements are frequently represented by signals, Transcription Start Site (TSS), -10 box DNA motifs. As such these representations are general and (GAGTAACAT) and -35 box (TTGACT) at 321 bp, 306 bp, can be used to describe any class of short DNA sequence 287 bp and three TF binding sites at 302 bp, 308 bp, 317 bp elements.
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